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KMID : 0358320090500080797
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Korean Journal of Urology
2009 Volume.50 No. 8 p.797 ~ p.804
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Protective Effect of Hyaluronic Acid on Cyclophosphamide-Induced Cystitis in Rats
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Yi Jeong-Won
Kim Youn-Hwa
Huh Hee-Jae
Lee Sun-Ju
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Abstract
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Purpose: We investigated the effect and duration of hyaluronic acid (HA) on the bladder of rats with cyclophosphamide (CYP)-induced cystitis.
Materials and Methods : CYP (250 mg/kg) was injected intraperitoneally in female Sprague-Dawley rats to induce cystitis. HA (0.5%) was instilled intravesically. First, the rats were separated into 2 groups for urodynamic study (CYP group, CYP＋HA group), and we assessed the inter-contraction interval, maximal voiding pressure (MVP), and pressure threshold of the 2 groups. Second, the rats were separated into 3 groups to assess the duration of effect of HA (CYP group; HA-1 group: HA was instilled 1 day before injection of CYP; HA-3 group: HA was instilled 3 days before injection of CYP), and we assessed the voiding interval for 7 days after confining the rats in metabolic cages.
Results: The inter-contraction interval of the CYP＋HA group (236.0±18.3 min) was significantly longer than that of the CYP group (178.0±13.4 min; p＜0.03), but the MVPs of both groups did not differ significantly (32.3± 1.2 vs. 34.0±2.4 cmH2O, respectively; p=0.56). The pressure threshold of the CYP+HA group (9.9±0.5 cmH2O) was significantly longer than that of the CYP group (13.2±0.7 cmH2O; p=0.002). The voiding interval decreased in each of the 3 groups according to the time after cystitis had been induced. The voiding interval was maximally decreased on the second day and returned to the pre-CYP injection level on the fifth day in each group. There was a significant difference in voiding interval among the 3 groups on the second day (p＜0.05). There was no significant difference between the CYP＋HA-1 and CYP＋HA-3 groups on the second day (p＞0.05).
Conclusions: HA has an effect of increasing the voiding interval in rats with CYP-induced cystitis, and the duration of effect is concluded to be 5 days. (Korean J Urol 2009;50:797-804)
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KEYWORD
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Hyaluronic acid, Cyclophosphamide, Cystitis
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